Sequencing Gels Not Sticking to Whatmann

Pascal Mertens Pascal.Mertens at fundp.ac.be
Fri Nov 7 11:52:15 EST 1997


In article (Dans l'article)
<kirkpat-0611971756420001 at gietz.hgen.umanitoba.ca>,
kirkpat at cc.?umanitoba?.ca (Rob Kirkpatrick) wrote (écrivait) :

>    One of the people in our lab is having a unique problem.  After running
> her sequencing gels she places the Whatman paper on the gel to remove it
> from the front plate and then disaster strikes.  The gel does not want to
> come off the glass plate and rips to shreds.  This has happened twice now
> and I can't figure it out.  The backplate is "Sigma Coated" so we can't
> really apply this to the front plate (or can we?).  We haven't changed the
> Whatman or anything else that I can think of...  Has anyone ever
> encountered this before???
> 
> Rob

You can put the first glass plate on the whatman and press to ensure
whatman paper is well coated with the gel. This sometimes helps!!

Pascal

-- 
Pascal Mertens
Laboratoire d'Immunologie et Microbiologie
URBM-FUNDP
61 Rue de Bruxelles
5000 Namur, BELGIUM
Phone: 32 81 724438
Fax: 32 81 724420



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