Prapering T-vecor and CIP

Kirov kirov at medfac.acad.bg
Mon Nov 10 01:35:29 EST 1997


Yoram Gerchman wrote:
> 
> While using T-vectors I have found a lot of times a self ligation
> problem.
> Is it posible to do CIP on the T-vector in order to avoid this problem?
> 
> .........................................................................
> Yoram Gerchman
> Division of Microbial & Molecular Ecology
> The Institute of Life Sciences
> Hebrew University of Jerusalem
> Givat Ram, Jerusalem 91904, Israel
> Tel: 972-2-6585175
NO.
Or you will have to phosphorylate your primers or PCR product.
-- 
Steven Kirov
PhD student
Lab. of Molecular Biology and Genetics
Dept. Of Biochemistry
Medical Faculty
Sofia
Bulgaria



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