activity of mammalian promoters in E.coli?
kuppig at immunbio.mpg.de
Mon Nov 10 06:56:17 EST 1997
the problem I faced recently was like this: I attempted to clone the cds of my
protein of interest into a mammalian expression vector and failed to get
positive E.coli clones. This protein (if expressed) is potentially toxic for
bacteria - even in small amounts. Does anybody know wheter the human beta
actin promoter (that´s the one my expression vector has) can drive expression
in E.coli? And what about other promoters (e.g. CMV)? Any suggestions are welcome!
Thanks in advance
Max-Planck-Institute for Immunobiology
D-79108 Freiburg, Germany
Email: kuppig at immunbio.mpg.de
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