Long PCR primers

Mikhail Alexeyev malexeye at JAGUAR1.USOUTHAL.EDU
Mon Nov 10 12:52:06 EST 1997


> Dear Colleagues,
>
> If there any risk of amplifying a fragment DNA with primer which are 50
> to 60 pb. Actually, I want to introduce a enzyme restriction site in
> these primers so that I can easily detected them in agore or Acrylamide
> gel. Therefore I do need longer primers. But I wandering about doing a
> PCR such long oligonucleotides.
> My regards,
>


I've  used primers up to 84 bp long with success.   Funny thing, though,
is that Vent does not seem to be willing to work with  longer primers
(unlike Tag) in my hands.

M.A.





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