House keeping gene for rat and guinea pig?

D. Pearton pearton at saul2.u.washington.edu
Mon Nov 10 19:17:49 EST 1997


Z. Huang (huangz at hotmail.com) wrote:
: Hi, bionetters,

: Now I am looking for primers for house keeping genes such as beta actin or
: GAPDH in rat and guinea pig. However, in case of guinea pig I can only find
: a short fragment of cDNA encoding GAPDH from genebank. And it's even worse
: that I can't find published sequence data for rat beta actin. My problem is:
: I want to design a set of primer to amplify cDNA of above house-keeping gene
: from rat, guinea pig. If it's possible this set of primers can also be used
: on human and rabbit. Is there anyone who know such a "universal primers for
: house keeping gene"? I would be very appreciate to any suggestions. Thanks.

I don't know about GAPDH but the primers for human RPO work fairly well
for rat cDNA.

Wasn't there some recent papers about variation in GAPDH levels anyway?

Cheers,
Dave
--
Dave Pearton
pearton at u.washington.edu
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Down he sank in a chair-ran his hands through his hair-	+
     And chanted in mimsiest tones			+
Words whose utter inanity proved his insanity,		+
     While he rattled a couple of bones.		+
							+
"The Hunting of the Snark" Lewis Carroll		+
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