Long PCR primers
khuang at BIOC.RICE.EDU
Mon Nov 10 14:27:21 EST 1997
My MegaPCR (300 bp, 600bp, 800bp) works fine!!
I used pfu and Taq, both are OK.
On 10 Nov 1997, Mikhail Alexeyev wrote:
> > Dear Colleagues,
> > If there any risk of amplifying a fragment DNA with primer which are 50
> > to 60 pb. Actually, I want to introduce a enzyme restriction site in
> > these primers so that I can easily detected them in agore or Acrylamide
> > gel. Therefore I do need longer primers. But I wandering about doing a
> > PCR such long oligonucleotides.
> > My regards,
> I've used primers up to 84 bp long with success. Funny thing, though,
> is that Vent does not seem to be willing to work with longer primers
> (unlike Tag) in my hands.
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