Long PCR primers

Ke-Xue Huang khuang at BIOC.RICE.EDU
Mon Nov 10 14:27:21 EST 1997


Hi, there,

My  MegaPCR (300 bp, 600bp, 800bp) works fine!!

I used pfu and Taq, both are OK.


Huang


On 10 Nov 1997, Mikhail Alexeyev wrote:

> > Dear Colleagues,
> >
> > If there any risk of amplifying a fragment DNA with primer which are 50
> > to 60 pb. Actually, I want to introduce a enzyme restriction site in
> > these primers so that I can easily detected them in agore or Acrylamide
> > gel. Therefore I do need longer primers. But I wandering about doing a
> > PCR such long oligonucleotides.
> > My regards,
> >
> 
> 
> I've  used primers up to 84 bp long with success.   Funny thing, though,
> is that Vent does not seem to be willing to work with  longer primers
> (unlike Tag) in my hands.
> 
> M.A.
> 
> 
> 



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