House keeping gene for rat and guinea pig?
Harry.Witchel at Bristol.ac.Uk
Tue Nov 11 07:04:00 EST 1997
. One way to test template quality is to use your template to amplify
glyceraldehyde phosphate dehydrogenase (GAPDH) using the primers:
GAPDH 248: ACC GGG AAG CCC ATC ACC &
GAPDH 672: CAG CCT TGG CAG CAC CAG
These produce a 400 bp fragment from most mammals (mouse rat rabbit human etc).
Z. Huang wrote:
> Hi, bionetters,
> Now I am looking for primers for house keeping genes such as beta actin or
> GAPDH in rat and guinea pig. However, in case of guinea pig I can only find
> a short fragment of cDNA encoding GAPDH from genebank. And it's even worse
> that I can't find published sequence data for rat beta actin. My problem is:
> I want to design a set of primer to amplify cDNA of above house-keeping gene
> from rat, guinea pig. If it's possible this set of primers can also be used
> on human and rabbit. Is there anyone who know such a "universal primers for
> house keeping gene"? I would be very appreciate to any suggestions. Thanks.
> Huang Zhong
> Gut Hormone Lab
> "Just keep trying...." ----Boss
Harry J. Witchel, Ph.D.
Bristol BS8 1TD
Harry.Witchel at Bristol.ac.uk
More information about the Methods