Gel extraction procedure

[Luc Gregoire]

Z. Huang wrote:
> 
> Tremblay,
> 
> I have been using a kit from invitrogene. It works well for me. I always
> check the yield by electrophoresis. I can always find visible or even strong
> band after gel purification. Anyway I would like suggest to use as much
> quantity of DNA as possible on the gel. I normally apply 30-40 ul DNA on
> well and gel purify them after electrophoresis. Then I use about 20 ul
> elution buffer to collect samples. 2ul of elution were put in the gel to
> check the yield of extraction. I have successfully used these elution in
> ligation, re-amplification by PCR, probe labelling without any problem. Good
> luck.
> 
> Huang zhong
> Gut Hormone Lab
> K.U.Leuven
> Belgium
> huang.zhong at med.kuleuven.ac.be
> 
> Guy Tremblay wrote in message <3467FEC1.779A at medcn.umontreal.ca>...
> >Hi,
> >
> >I am using a kit for gel extraction of DNA bands. This kit, although
> >packaged in a beautiful box, has low yields and I am now inclined to
> >throw this box away - even though it has so nice colors. I wish they
> >put their effort on something else than the packaging.
> > So does anyone have a clean method to do gel extracts that have good
> >yields, some kind of an old fashion way that would be clean enough for
> >blunt-ended ligations? Or even a kit with high yields and an ugly
> >packaging?
> >
> >Thanks in advance and have good experiments (without kits).
> >
> >-----------------------------------
> >Guy Tremblay
> >Universite de Montreal
> >tremblgu at magellan.umontreal.ca!!sorry, It should be a kit from Boeringer-Manheim (Agarose gel 
purification kit).

Z.Huang