Homogeneous plasmid population?

Koen De Smet k.desmet at nospam.ic.ac.uk
Tue Nov 11 09:42:07 EST 1997

Harry Witchel wrote:
>     One possibility is that my plasmid is really a population of two
> plasmids which are similar but not identical.  How do I guarantee that I
> have only one plasmid species in my transfections, and how would I
> purify out one plasmid if I did indeed have two plasmids in my
> population.
>     Thanks,
>         Harry
Put the plasmid(s) back in E. coli and pick single colonies. They will 
have either the 'right' or 'wrong' plasmid. It is up to yuo to find out 
which colony has which plasmid...

Koen De Smet
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     Imperial College School of Medicine at St Mary's   

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