Long PCR

mehboob shivji shivjm01 at mcrcr.med.nyu.edu
Tue Nov 11 22:43:39 EST 1997


I am having a problem amplifying a 2700 bp fragment.
Anneal time = 2 min @ 55 deg
Elongation = 3 min @ 72 deg
Denature = 2 min @ 95 deg
I would appreciate any suggestions.



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