DNA in invertebrates HELP!
jladasky at cmgm.Stanford.EDU
Tue Nov 11 19:13:25 EST 1997
In article <Pine.SOL.3.96.971111170752.21365A-100000 at suma3.reading.ac.uk>,
Clive Boorman <sau95cb at reading.ac.uk> wrote:
>Hi anyone who can help,
> We have been looking at invertebrate crustacea genes and have
>isolated a cDNA sequence for the gene in question. Our problem is, getting
>any further. The genomic DNA is causing us many problems probably caused by
>polysaccharides. Having extracted DNA from whole tissue and purified it we
>have to re-extract it using CTAB, however this has not cured our problems.
>Primers which work on mRNA don't work on genomic DNA.
> Has anyone been working on DNA extraction and analysis in
>invertebrates who can help solve the possible polysaccharide or other
>peculiarities of crustacean DNA ?
>Is there any other reason why we may be getting no PCR product from
>genomic DNA, when we can get a product from mRNA (RT-PCR) everytime ?
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