Long PCR

Zhongtang Yu zyu at UNIXG.UBC.CA
Wed Nov 12 12:18:46 EST 1997


You might want to try 30 sec annealing and 30 or 45 sec denaturing.

Z. Yu.
Microbiol, UBC.


On Tue, 11 Nov 1997, mehboob shivji wrote:

> I am having a problem amplifying a 2700 bp fragment.
> Anneal time = 2 min @ 55 deg
> Elongation = 3 min @ 72 deg
> Denature = 2 min @ 95 deg
> I would appreciate any suggestions.
> 
> 




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