Sequencing with NP-40

Thomas urbig at
Fri Nov 7 06:25:26 EST 1997


I used to do the sequencing reaction (Sequenase Protocool) as described in
Hutchinson and Dahl (Biotechniques 19: 554-556 (1995)) with NP-40 instead
of alkaline denaturation as a first step. Unfortunately NP-40 is no longer
available from Sigma. Does anybody know a source for NP-40 or has tried
the substitute IGEPAL CA-630 that is now offered from sigma as a

Thanks in advance


Thomas Urbig
email: urbig at

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