A-overhang for TA cloning

Frederik Boernke boernke at ipk-gatersleben.de
Thu Nov 13 11:10:31 EST 1997

umrafajl at cc.umanitoba.ca wrote:
> Sometime back I read in this group that the base composition (A, G, T,
> or C) of the last base of a PCR product could affect the addition of
> "A". I forgot which one favor the formation of the "A"-overhang. I'll
> appreciate any information. You post reply here or email me at
> h_y at hotmail.com.  Thanks
> HY


As far as I remember it was an A which reduces cloning efficiencies by
20 - 40%, but I never made this experience.



Frederik Boernke
Research Group of  Molecular Plant Physiologie
Institute for Plant Genetics and Crop Plant Research (IPK)
Corrensstr. 3
06466 Gatersleben
Tel.  039482 -5 321
Fax. 039482 -5 515
e-mail: boernke at ipk-gatersleben.de

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