Sequencing Gels Not Sticking to Whatmann

Bill Geese wjgeese at astro.ocis.temple.edu
Wed Nov 12 14:59:24 EST 1997


Try fixing it in 10% acetic acid for 30min to remove the urea, then 
transferring it.  Sometimes that loosens them up.

Bill

> 
> 
> 
> >    One of the people in our lab is having a unique problem.  After
> >    running
> > her sequencing gels she places the Whatman paper on the gel to
> > remove it from the front plate and then disaster strikes.  The gel
> > does not want to come off the glass plate and rips to shreds.  This
> > has happened twice now and I can't figure it out.  The backplate is
> > "Sigma Coated" so we can't really apply this to the front plate (or
> > can we?).  We haven't changed the Whatman or anything else that I
> > can think of...  Has anyone ever encountered this before???
> > 
> > Rob
> > 
> > 
> > remove the ??'s from e-mail to reply
> > 
> > -- 
> > 
> > 
> > 
> > 
> -----
> usual disclaimers apply
> -----                              
> Wolfgang Schechinger         
> University of Tuebingen
> email: wgschech at med.uni-tuebingen.de
> http://www.medizin.uni-tuebingen.de/~wgschech/research.htm
> -------
> !Junk mail is *not* appreciated!
> SPAMs will be answered automatically by sending my 128MByte virtual memory file.
> This is *NO* joke.
> 
> 
> 



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