Titre of PF-BOS cDNA library

L.D. Ofner BMBLDO at leeds.ac.uk
Thu Nov 13 08:36:44 EST 1997


Dear all,

I want to pull out a full length clone from a cDNA library using PCR. The 
library is constructed in the PF-BOS expression vector and I'm uncertain how 
to titre and amplify the library. I know that with a phage library you infect 
bacteria and utilise the lysogenic cycle of the phage. Would it just be a 
case of propagating the clones in bugs etc ? I'd be very grateful for a 
detailed protocol or any advice that you can offer.


Thanks,

Lisa (bmbldo at leeds.ac.uk)         



More information about the Methods mailing list