Ligation Product is Shoter than Vector

ma ddsyr at PUBLIC.EAST.CN.NET
Thu Nov 13 06:54:30 EST 1997


Dear netters:
At present, I clone a PCR product. When I ligate the PCR product into pGEM7Z vector, I got unusual result.
It should have two kind products. One is vector plus insert, one is vector alone. But I got the third kind
product, its size is shorter than vector after a single enzyme cut (say HindIII, which has unique site in
pGEM7Z). I wonder the reason. Anyone have this kind of experience and kindly give some information.
Ma




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