Sequencing Gels Not Sticking to Whatmann

Mart Speek mspeek at tamm.eenet.ee
Thu Nov 13 03:27:48 EST 1997


Hey Sequencing Folks!

(problem: gel doesn't stick to the Whatman 3M paper!?)

Possible cause/solution:

# I have encountered similar problem when the PAAG 
percentage was 10% or higher (as % was not mentioned in the
original message, I assume it was higher than 5%)
My guess is that this may be due to insufficient wetness of
the gel; slight prewetting of the paper would help
(at least it worked for me!)

Mart (mspeek at ebc.ee in ESTONIA)  



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