A-overhang for TA cloning

Dr. Duncan Clark duncan at genesys.demon.co.uk
Sat Nov 15 05:01:21 EST 1997

In article <346A799B.1984 at cc.umanitoba.ca>, umrafajl at cc.umanitoba.ca
>Sometime back I read in this group that the base composition (A, G, T,
>or C) of the last base of a PCR product could affect the addition of
>"A". I forgot which one favor the formation of the "A"-overhang. I'll
>appreciate any information. You post reply here or email me at
>h_y at hotmail.com.  Thanks 

I put such a post quite a while ago. I can't remember the exact
reference other than Biotechniques, I think last year. Try searching the
archives for my past posts (there will be a lot so it may take a

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
TEl/FAX 01252376288

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