two bands after fill-in

daij at okstate.edu daij at okstate.edu
Mon Nov 17 20:30:07 EST 1997


How is your DNA insert prepared? Digestion-heat inactivation of RE? Your
might denature you insert.

On 16 Nov 1997, Mr. G. Anil Kumar wrote:

> 
> Dear friends,
> I am facing a very funny problem. When I am using a particular DNA insert
> for fill-in, after the fill in I am seeing two bands in the gel. This is
> repeatedly happening with only this template though I rountinely do this
> fill in with different templates without any problems. 
> 
> Thanks
> anil
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> G.ANILKUMAR
> MICROBIOLOGY AND CELL BIOLOGY 
> INDIAN INSTITUTE OF SCIENCE
> BANGALORE 560 012
> INDIA.
> Tel.0091-080-3092344
> Fax. 0091-080-3444697
> 
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> 




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