A-overhang for TA cloning

David F. Spencer dspencer at is.dal.ca
Mon Nov 17 15:50:54 EST 1997


In article <zX8UkTAxLXb0Ewmd at demon.co.uk>, "Dr. Duncan Clark"
<duncan at genesys.demon.co.uk> wrote:

> In article <346A799B.1984 at cc.umanitoba.ca>, umrafajl at cc.umanitoba.ca
> writes
> >Sometime back I read in this group that the base composition (A, G, T,
> >or C) of the last base of a PCR product could affect the addition of
> >"A". I forgot which one favor the formation of the "A"-overhang. I'll
> >appreciate any information. You post reply here or email me at
> >h_y at hotmail.com.  Thanks 
> >
> >HY
> 
> 
> I put such a post quite a while ago. I can't remember the exact
> reference other than Biotechniques, I think last year. Try searching the
> archives for my past posts (there will be a lot so it may take a
> while!).
> 
> Duncan


Brownstein et al. 
Modulation of Non-Templated Nucleotide Addition by Taq DNA Polymerase:
Primer Modifications that Facilitate Genotyping.
BioTechniques 20(6): 1004-1010 (1996)

A very useful paper.

Dave

--------------------------------------------
David F. Spencer, PhD
Dept. Of Biochemistry
Dalhousie University
Halifax, Nova Scotia
Canada

dspencer at is.dal.ca
dspencer at rsu.biochem.dal.ca



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