How to protect oligo from 5' exo in cellular extract

Olivier Becherel Olivier.Becherel at esbs.u-strasbg.fr
Mon Nov 17 11:02:57 EST 1997


I use an 24mer oligonucleotide in primer elongation reaction catalyzed 
by extract from eucaryotic cells. This primer is labelled in 5' by 
kination with 32P but during the incubation, this label is lost probably 
by the action of phosphatase and exonuclease from the extract.
If somebody have some experience in protecting this extremity thank you 
for your help

Thanks

Please reply to:

Agnes CORDONNIER
E-mail: Agnes.Cordonnier at esbs1.u-strasbg.fr



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