cDNA from bacteria?
mantei at neuro.biol.ethz.ch
Mon Nov 17 10:04:19 EST 1997
In article <trevor-ya02408000R1611971312320001 at nntp.stanford.edu>,
trevor at leland.stanford.edu (Trevor Bezdek) wrote:
> Is there any way to make cDNA from bacterial mRNA? The lack of a polyA
> tail seems daunting but is there any way to use linkers or some modified
> anchor oligos to achieve this?
Besides the lack of poly(A), the half-life of bacterial mRNA is so short
that the 5'-end may be degraded before the 3'-end has even been
Dept. of Neurobiology, Swiss Federal Institute of Technology
CH-8093 Zurich, Switzerland
mantei at neuro.biol.ethz.ch Fax: +41-1-633-1046
More information about the Methods