cDNA from bacteria?

Ned Mantei mantei at neuro.biol.ethz.ch
Mon Nov 17 10:04:19 EST 1997


In article <trevor-ya02408000R1611971312320001 at nntp.stanford.edu>,
trevor at leland.stanford.edu (Trevor Bezdek) wrote:

> Is there any way to make cDNA from bacterial mRNA?  The lack of a polyA
> tail seems daunting but is there any way to use linkers or some modified
> anchor oligos to achieve this?  

Besides the lack of poly(A), the half-life of bacterial mRNA is so short
that the 5'-end may be degraded before the 3'-end has even been
synthesized.

-- 
Ned Mantei
Dept. of Neurobiology, Swiss Federal Institute of Technology
CH-8093 Zurich, Switzerland
mantei at neuro.biol.ethz.ch   Fax: +41-1-633-1046



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