RNA sequencing with dye-deoxy-NTP's

Philip Buzby phil.buzby at usa.dupont.com
Mon Nov 17 07:25:37 EST 1997


This is a multi-part message in MIME format.

------=_NextPart_000_0004_01BCF32A.27A91660
Content-Type: text/plain;
	charset="iso-8859-1"
Content-Transfer-Encoding: quoted-printable

The enzyme RT (AMV) was one of the first enzymes (Sequenase the second) =
which were originally used in dye-dideoxyNTP DNA sequencing.  Therefore =
to sequence RNA, RT is a viable choice.  For questions read:  Prober et =
al Science 238, 336-341 (1987).

------=_NextPart_000_0004_01BCF32A.27A91660
Content-Type: text/html;
	charset="iso-8859-1"
Content-Transfer-Encoding: quoted-printable

<!DOCTYPE HTML PUBLIC "-//W3C//DTD W3 HTML//EN">
<HTML>
<HEAD>

<META content=3Dtext/html;charset=3Diso-8859-1 =
http-equiv=3DContent-Type>
<META content=3D'"MSHTML 4.71.1712.3"' name=3DGENERATOR>
</HEAD>
<BODY bgColor=3D#ffffff>
<DIV><FONT color=3D#000000 size=3D2>The enzyme RT (AMV) was one of the =
first enzymes=20
(Sequenase the second) which were originally used in dye-dideoxyNTP DNA=20
sequencing.&nbsp; Therefore to sequence RNA, RT is a viable =
choice.&nbsp; For=20
</FONT><FONT color=3D#000000 size=3D2>questions read:&nbsp; Prober et al =
Science=20
238, 336-341 (1987).</FONT></DIV></BODY></HTML>

------=_NextPart_000_0004_01BCF32A.27A91660--




More information about the Methods mailing list