Mini Agarose Gel Problem

Prince LoLo g2bfunky at ix.netcom.com
Tue Nov 18 01:33:35 EST 1997


I am having problem resolving DNA on a mini (5cm x 8cm x 3mm) agarose
gel. 1X TBE.  The bands are slanted, such that the DNA closer to the
top gel layer travels slower than DNA closer to the bottom layer.
Observing the gel from the side, the bands shape like a
right-side-left "C".  Slowing the running condition to 1 V/cm or
reducing the sample did not remedy the situation.

Can anyone speculate the probable cause of the problem?

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