mRNA isolation?

Alan Lee ywlee at hkucc.hku.hk
Tue Nov 18 22:01:19 EST 1997


Hello everybody,
We are trying to isolate mRNA from total RNA (from mouse tissues).
Though there are various types of mRNA purification kit available
commercially, we have no idea about their efficiency in isolating
high-molecular weight mRNA (we are interested in RNA of transcript size
~11 kb). Anyone who have experience on this aspect please kindly drop me
an e-mail. Thank you in advance.
Alan Lee
e-mail: ywlee at hkucc.hku.hk

P.S. Any comments on PolyAtract from Promega or mRNA isolation kit from
BM.



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