kpmitton at umich.edu
Tue Nov 18 12:21:27 EST 1997
We use Sequagel, gel solutions (gel concentrate and gel diluent) with
our own gel buffer added.
Let your gels set for at least 2 hours, we typically make them the day
Mix the gel mix thoroughly, I add the amm. pers. last, from a 10%
If your gel is not even, then you may have local areas heating up, is
the backing heat transfer plate on your gel apparatus still smooth or
bumpy and rusted out?
More information about the Methods