DNA contamination in PCR

Jean-Christophe Roux jcroux at rockefeller.univ-lyon1.fr
Tue Nov 18 09:50:43 EST 1997


Dear all,
I try to extract RNA from brain but I have a big contmination(DNA).
Its special with brain because i have good results with other
tissues(muscle,liver...).
My problem is at the first remove of aqueous phase, the pellet is not
hard, compact.
Is it necessary to use DNAse?
Thank you very much!




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