Non-specific signals from BAC pools

Bernard Konfortov bkonfort at hgmp.mrc.ac.uk
Wed Nov 19 12:37:08 EST 1997


Hi,

I have a dissapointing experience with a BAC library. I want to isolate
a clone by PCR screening of a superpool, but all the time I am getting
products of a simmilar size from different pools. I am working at the
highest possible annealing temperature, my water control sample is
always blank, and PCR with vector only and with bacterial DNA did not
produce any signal. Has anyone else experienced similar problems?

Bernard



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