reuse of Southern blots

someone in the lab wormlab at ncsu.edu
Wed Nov 19 08:16:01 EST 1997


Marisol Rodriguez Pena wrote:
> 
> Hi there,
> I reused nylon membranes twice with succes whith the following protocol:
> Heat 0.1xSSC/0.1%SDS to boil
> Whash 2x15 min ...
> Proceed with prehybridization...

Quick question - is it really neccessary to pre-hyb after the first 
one? Can't another probe be added right after washing?

Please reply in newsgroup - the address above is bogus.



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