reuse of Southern blots

Marisol Rodriguez Pena pena at chem.vu.nl
Wed Nov 19 07:31:21 EST 1997


Hi there,
I reused nylon membranes twice with succes whith the following protocol:
Heat 0.1xSSC/0.1%SDS to boil
Whash 2x15 min (or more, you can check if there is radioactivity in your 
gel if you rehybridize soon after the first hybridization)
Proceed with prehybridizatio or store the membrane in plastic wrap, 
dissecated.
Hope it helps


Greatings


Marisol
-- 
MS Rodriguez Pena, MD, phD
Department of Pharmacochemistry, Faculteit der Scheikunde
Vrije Universiteit. 
De Boelelaan 1083,
1081 HV Amsterdam, The Netherlands
Tel: 31-20-4447572
Fax: 31-20-4447610



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