PCR witg high GC

Paul Monis pmonis at g.adelaide.edu.au
Thu Nov 20 00:06:17 EST 1997


Have you tried including DMSO in the reaction? I've used up to 10% DMSO
(final conc.) to successfully amplify high GC regions. I'd imagine it would
be alot cheaper to do it this way if you could.
Cheers

Paul Monis





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