Dr. Duncan Clark
duncan at genesys.demon.co.uk
Wed Nov 19 10:06:37 EST 1997
In article <Pine.SOL.3.96.971119102301.28176A-
100000 at suma3.reading.ac.uk>, Clive Boorman <sau95cb at reading.ac.uk>
>During extraction of DNA from crustacea we have found an oily DNA
>precipitate (after pelleting) rather than normal wool-like precipitate.
>From Gill it is OK, but from muscle and mid-gut gland it is oily. The
>same purification methods are used in both cases. Any suggestions why we
>are getting oily DNA (regardless of how many time we wash in ethanol) ?
We've found this several times with various hyperthermophiles, but only
those grown in sea-water media. It's never stopped anything working so
we tend not to worry anymore.
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
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