Oily DNA

Dr. Duncan Clark duncan at genesys.demon.co.uk
Wed Nov 19 10:06:37 EST 1997


In article <Pine.SOL.3.96.971119102301.28176A-
100000 at suma3.reading.ac.uk>, Clive Boorman <sau95cb at reading.ac.uk>
writes
>During extraction of DNA from crustacea we have found an oily DNA
>precipitate (after pelleting) rather than normal wool-like precipitate.
>From Gill it is OK, but from muscle and mid-gut gland it is oily. The
>same purification methods are used in both cases. Any suggestions why we
>are getting oily DNA (regardless of how many time we wash in ethanol) ? 


We've found this several times with various hyperthermophiles, but only
those grown in sea-water media. It's never stopped anything working so
we tend not to worry anymore.

Duncan

 
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
TEl/FAX 01252376288
http://www.dnamp.com
http://www.genesys.demon.co.uk



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