Preparation of COS7 cells for FACS analysis
M. Johan Broekman, PhD
mjbroek at mail.med.cornell.edu
Mon Nov 24 21:14:10 EST 1997
We have no problems with lifting the cells with EDTA/BSA, especially if they
have only grown for 1-2 days. Try putting the dish on wet ice, and light
scraping with a "rubber policeman".
Jun-Ichi Aikawa wrote:
> Hello. Does anyone try to prepare COS7 cells for analysis of cell-surface
> antigen with FACS? When I treated cells with PBS/5mM EDTA(pH 7.2) to
> prepare single-cell suspension, the efficiency is nearly 20-40% and most of
> cells still remains undispersed. I also tried the increase of EDTA
> concentration (up to 25mM) and the addition of BSA(1%), but no improvement
> was observed. What buffer is suitable for this study? Any comments and
> suggestions will welcome.
> Jun-ichi Aikawa, Ph.D.
> University of California, San Diego
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