16S rDNA PCR

Julie Proffitt Julie.Proffitt at dfst.csiro.au
Tue Nov 25 01:37:35 EST 1997


I am trying to develop a PCR detection test for a microorganism using
specific primers based on variable regions of the 16S gene.  I am having
problems getting product at low template concs (<1ng/rxn).  I believe the
problem may be partly due to secondary structure formation in the template
during annealing.  I have tried adding DMSO to the mix which has helped but
I still have poor results.  Has anyone had any success with a similar PCR or
have any suggestions?

Thanks






More information about the Methods mailing list