B. subtilis and cat genes
pcmoe at students.wisc.edu
Mon Nov 24 09:38:47 EST 1997
I am attempting to disrupt a B. subtilis gene with an insertion
carrying a cat gene that has worked quite well in E. coli. My first round of
transformation yielded no Cm resistant recombinants. My question is: will a
cat gene that works in gram negatives necessarily work in Bacillus?
TIA for any help you folks can render in this!
Paul C. Moe
University of Wisconsin-Madison
Laboratory of Molecular Biology
305 R.M. Bock Laboratories
1525 Linden Drive
Madison, WI 53706-1596
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