B. subtilis and cat genes

[Paul Moe]

Dear all,
        I am attempting to disrupt a B. subtilis gene with an insertion 
carrying a cat gene that has worked quite well in E. coli. My first round of 
transformation yielded no Cm resistant recombinants. My question is: will a 
cat gene that works in gram negatives necessarily work in Bacillus?

        TIA for any help you folks can render in this!




Paul C. Moe
University of Wisconsin-Madison
Laboratory of Molecular Biology
305 R.M. Bock Laboratories
1525 Linden Drive
Madison, WI  53706-1596
USA
Lab:(608) 262-7976
FAX: (608)262-4570