The acidity problem in the two-hybrid system

Per-Anders Olsson Pandol at bmc.uu.se
Tue Nov 25 17:01:40 EST 1997


What is the minimum number of local acidic amino acids, D´s and E´s, that
can give self activation of a bait in the two-hybrid system, in spite of
an acceptable average isoelectric point for the whole bait peptide?



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