NdeI versus NcoI... which is best?

Stuart Rison stuart at ludwig.ucl.NO_JUNK.ac.uk
Wed Nov 26 10:39:01 EST 1997

Dear All,

I am curently developing a cloning scheme in which I want to use a RE that
incorporates a start (ATG) codon.

I've narrowed down my RE list to NdeI or NcoI (although this purely through
hearsay/gut-feeling so please do tell me if you think I've missed out a good

Does anybody have any experience using these REs?  Which one works best? 
Have you ever had any trouble with them?

Looking forward to hearing any suggestions.



| Stuart Rison            | Ludwig Institute for Cancer Research |   
| Tel. (0171) 878 4127    | Courtauld Building                   | 
| Fax. (0171) 878 4040    | 91 Riding House Street               |
+-------------------------+ London, W1P 8BT                      |
| stuart at ludwig.ucl.ac.uk | UNITED KINGDOM.                      |
- and there is a NO_JUNK bit to remove in my return address !!!! -

More information about the Methods mailing list