Protein precipitation

Martin Offterdinger a8803349 at
Thu Nov 27 03:44:08 EST 1997

On Wed, 26 Nov 1997 22:07:23 -0500, pxpst2 at
(Peter) wrote:

>In article <347B264C.1B6C at>, rdudley at wrote:
>> I need to concentrate a GST fusion protein I have purified.  I have seen
>> a number of purification schemes--methanol/chloroform, acetone, TCA,
>> etc--but very few ideas on how to resuspend the precipitated protein. 
>> Does anyone have a favorite method, or could give an idea on good
>> buffers in which to redissolve the precipitated protein?
>Do not precipitate the protein.  Extract it utilizing phenol and ether.
>pxpst2 at vms.cis.pitt.ed.u
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What about using a microconcentrator that can be centrifuged- e.g.
Amicon microconcentrators- a very simple method of ultrafiltration
that does not cause denaturation of your protein.

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