Protein precipitation

Martin Offterdinger a8803349 at unet.univie.ac.at
Thu Nov 27 03:44:08 EST 1997


On Wed, 26 Nov 1997 22:07:23 -0500, pxpst2 at vms.cis.pitt.delet.edu
(Peter) wrote:

>In article <347B264C.1B6C at nrc.uab.edu>, rdudley at nrc.uab.edu wrote:
>
>> I need to concentrate a GST fusion protein I have purified.  I have seen
>> a number of purification schemes--methanol/chloroform, acetone, TCA,
>> etc--but very few ideas on how to resuspend the precipitated protein. 
>> Does anyone have a favorite method, or could give an idea on good
>> buffers in which to redissolve the precipitated protein?
>
>
>Do not precipitate the protein.  Extract it utilizing phenol and ether.
>
>Peter
>pxpst2 at vms.cis.pitt.ed.u
>
>-- 
>"Don't you eat that yellow snow
>    WAtch out where the Huskies go"
>
>---------------------------------------------------------------------
>
>Let us not forget those marketing boys from SPAM CENTRAL
>
>bestrealtor at marketingmaster.com
>info at herbchew.com
>clickthru at timefreedom.com
>invite at onlinenow.net
>zippydj at nevwest.com
>Offer at shire.com
>empower at empowerlabs.com
>dynamarket at vaprnet.com
>root at mail.icongrp.com
>cashrewards at hotmail.com
What about using a microconcentrator that can be centrifuged- e.g.
Amicon microconcentrators- a very simple method of ultrafiltration
that does not cause denaturation of your protein.
martin



More information about the Methods mailing list