Protein precipitation

Peter pxpst2 at
Wed Nov 26 22:07:23 EST 1997

In article <347B264C.1B6C at>, rdudley at wrote:

> I need to concentrate a GST fusion protein I have purified.  I have seen
> a number of purification schemes--methanol/chloroform, acetone, TCA,
> etc--but very few ideas on how to resuspend the precipitated protein. 
> Does anyone have a favorite method, or could give an idea on good
> buffers in which to redissolve the precipitated protein?

Do not precipitate the protein.  Extract it utilizing phenol and ether.

pxpst2 at vms.cis.pitt.ed.u

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