Protein precipitation

Peter pxpst2 at vms.cis.pitt.delet.edu
Wed Nov 26 22:07:23 EST 1997


In article <347B264C.1B6C at nrc.uab.edu>, rdudley at nrc.uab.edu wrote:

> I need to concentrate a GST fusion protein I have purified.  I have seen
> a number of purification schemes--methanol/chloroform, acetone, TCA,
> etc--but very few ideas on how to resuspend the precipitated protein. 
> Does anyone have a favorite method, or could give an idea on good
> buffers in which to redissolve the precipitated protein?


Do not precipitate the protein.  Extract it utilizing phenol and ether.

Peter
pxpst2 at vms.cis.pitt.ed.u

-- 
"Don't you eat that yellow snow
    WAtch out where the Huskies go"

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