NdeI versus NcoI... which is best?

Keith Jolley K.Jolley at soton.ac.uk
Wed Nov 26 11:34:13 EST 1997


I've used them both without any problems (purchased from NEB).  They
both have the same buffer requirements so that needn't be a
consideration.  While neither are particulary expensive (relative to
other REs), NdeI seems to be cheaper.

On Wed, 26 Nov 1997 15:39:01 GMT, "Stuart Rison"
<stuart at ludwig.ucl.NO_JUNK.ac.uk> wrote:

>Dear All,
>
>I am curently developing a cloning scheme in which I want to use a RE that
>incorporates a start (ATG) codon.
>
>I've narrowed down my RE list to NdeI or NcoI (although this purely through
>hearsay/gut-feeling so please do tell me if you think I've missed out a good
>RE).
>
>Does anybody have any experience using these REs?  Which one works best? 
>Have you ever had any trouble with them?
>
>Looking forward to hearing any suggestions.
>
>Cheers,
>
>Stuart.


-- 
Dr. Keith Jolley
Dept. of Molecular Microbiology
Southampton General Hospital, UK
Tel: 01703-798895  Fax: 01703-774316



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