Where is the promotor of he tetR gene on pBR322?

Richard L lm11 at gpu.srv.ualberta.ca
Fri Nov 28 14:43:54 EST 1997


Hi Yoram,

The promoter site of pBR322 tet is in the HindIII region. So if you want
cut the whole gene with a functinal promoter, you'd better use EcoRI, not
HindIII. 

Richard



Yoram Gerchman <yoramg2 at LEONARDO.LS.HUJI.AC.IL> wrote:
: Happy thanksgiving to every body, and a question. Does anyone know where is
: the promoter site of the tet resistance gene of pBR322?

: .........................................................................
: Yoram Gerchman
: Division of Microbial & Molecular Ecology
: The Institute of Life Sciences
: Hebrew University of Jerusalem
: Givat Ram, Jerusalem 91904, Israel
: Tel: 972-2-6585175




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