One tube RT-PCR

Giuseppe Palumbo gap at
Sat Nov 22 20:52:43 EST 1997

Does anyone have tryed one tube rt-PCR with good results?
I have some trouble with Tth and I heard about MuLV-RT + Taq Gold.
How much RNA, primers, Mg, etc.
Please respond also by e-mail to:
gap at
Thank you,

Giuseppe Palumbo, M.D.
Institute of Hematology
University of Catania, Italy

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