Primer Degradation?

Z. Zhao zxz4 at PSU.EDU
Wed Oct 1 10:31:04 EST 1997


You suspect that degraded primers were used as primers and 
the original oligos were used as templates in your PCR. How 
long is the largest PCR product in your water controls? If 
it is longer than 25 bp, I would guess that your PCR was 
contaminated, which might be in your primer stock.



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