in situ using oligos

cgriffin at wco.com cgriffin at wco.com
Wed Oct 1 22:35:48 EST 1997


Hello Ashish,

Frankly  I would suggest going with a  cRNA probe, following
hybridization, you can use RNAse to go a long way to lowering  the
background. If you want info on protocols with cRNA  probes let me know. 

Ciao,

Chandi Griffin 
UCSF/SFGH





In article
<Pine.A41.3.95.970915171036.93558B-100000 at black.weeg.uiowa.edu>, "A.
Kumar" <akumar at blue.weeg.uiowa.edu> wrote:

> I am trying in-situ hybridization using oligonucleotide probes labeled
> with S35. I have tried a couple times, but I either get too high
> background, or no signal at all. I am using 2-mercaptoethanol, and sodium
> thioshulfate in washes, and DTT in the hybridization buffer. I have
> referred to a number of publications for methods, and seem to be doing the
> right thing. Does anyone have any suggestions or ideas where I might get a
> better protocol?
> thanks
> 
> 
> Ashish Kumar
>  
>                    WORKPLACE                      DEN
>                 
>         Dept. of Anatomy and Cell Biology,     735 Michael St. Apt 34
>         College of Medicine,                   Iowa City
>         University of Iowa,                    IA 52246-5523
>         Iowa City, IA 52242
> 
>         Dial (319)335-7739                     (319)338-0529
> 
>                 e : ashish-kumar at uiowa.edu
> 
> 
>



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