woodlab at SUNGCG.USOUTHAL.EDU
Wed Oct 1 16:58:56 EST 1997
Hello out there,
I am trying to determine if there is any methylation in the
bacterial system I am using. So far, I have done the straight-forward
restriction digestions, using HinfII, etc. No methylation detected. I
have been reading about the bisulfate modification of the cytosines and
I'll probably give it a try. My concern is that my critter is very A/T
rich and only about .75% of E. coli's Cytosines are methylated. I am
afraid I'll miss it. I have also noted that someone determined 1% of
the adenines of E. coli are methylated. How did they determine that??
If I could check for methylation of adenine, that would be great.
If any of you have information on this or any methods I haven't heard of,
I would greatly appreciate it. Oh, Mass-spec-Mass-spec may be out of the
question, but I'll take info or references on that as well.
Thanks in advance,
Department of Microbiology
University of South Alabama
e-mail woodlab at sungcg.usouthal.edu
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