Long PCR

Albé van der Merwe ZA vdmerwea at intekom.co.za
Wed Oct 1 14:53:24 EST 1997


Rajesh Kumar <rk6n at virginia.edu> wrote in article
<3432715B.72BA at virginia.edu>...
> Hi,
> 
> I need to do a long PCR (~9 kb). Can somebody suggest me a suitable
> polymerase and a source of it ? How about using a mixture of two
> polymerases, say, Taq and Pfu ?
> 
> Any suggestion will be highly appreciated.
> 
> Rajesh
> 

Try Expand mixture. It has Pwo, and Taq (I think). Annealing temp. should
be 68*C for >3 kb, and 72*C for <3 kb. Hope this helps.

[:)



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