restriction digestion of PCR product for cloning

Matthew Knight s9401501 at COUGAR.VUT.EDU.AU
Thu Oct 2 02:37:12 EST 1997


I am having problems digesting a PCR product which has restriction enzyme
sites placed into the PCR primers, thus they are very close to the end of
the PCR product.

Does anyone have any suggestions on how I can be sure that the
restriction enzyme is going to cut the PCR product. And will the
restriction enzyme cut with the sequence being so close to the end of the
product (i.e. about 5 or 6 bases)

Thanks in advance

Matthew Knight




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