restriction digestion of PCR product for cloning
zinc at genetics.utah.edu
Thu Oct 2 12:52:34 EST 1997
the NEB catalog covers this quite nicely. it's always good to have
more at the ends, ie. order longer primers.
s9401501 at COUGAR.VUT.EDU.AU (Matthew Knight) writes:
> I am having problems digesting a PCR product which has restriction enzyme
> sites placed into the PCR primers, thus they are very close to the end of
> the PCR product.
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Karl Shapiro,(1959) from an essay on Henry Miller's Tropic of Cancer
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