Primer Degradation?

Pascal Mertens Pascal.Mertens at fundp.ac.be
Wed Oct 1 03:41:49 EST 1997


In article (Dans l'article) <gilbride-3009971316320001 at ppp-10x1-5.bu.edu>,
gilbride at bu.edu (Kevin Gilbride) wrote (écrivait) :

> I need some advise regarding the stabilty of primers.

Not directly linked to your problem:
long term (in)stability: a (previously good) primer stored 6 years at -20
as a 100X stock and reused in sequencing does not work anymore.
It seems to be the same for a plasmid (maxiprep, 6 years old): no more
readable sequence (tested of course with a new primer that works on a new
DNA).

Pascal

-- 
Pascal Mertens
Laboratoire d'Immunologie et Microbiologie
URBM-FUNDP
61 Rue de Bruxelles
5000 Namur, BELGIUM
Phone: 32 81 724438
Fax: 32 81 724420



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